» Deep phenotyping of the lipidomic response in COVID-19 and non-COVID-19 sepsis
From pubmed: wherry ej[au]
CONCLUSIONS: A broad based lipidomic storm is a predictor of poor prognosis in ARDS. Alterations in sPLA2, PGD2 and 12-HETE and the high abundance lipids, ChoE 18:3, LPC-O-16:0 and PC-O-30:0 exhibit relative specificity for COVID-19 amon...
» Serotonin reduction in post-acute sequelae of viral infection
From pubmed: wherry ej[au]
Post-acute sequelae of COVID-19 (PASC, "Long COVID") pose a significant global health challenge. The pathophysiology is unknown, and no effective treatments have been found to date. Several hypotheses have been formulated to explain the ...
» Trimodal single-cell profiling reveals a novel pediatric CD8αα+ T cell subset and broad age-related molecular reprogramming across the T cell compartment
From pubmed: wherry ej[au]
Age-associated changes in the T cell compartment are well described. However, limitations of current single-modal or bimodal single-cell assays, including flow cytometry, RNA-seq (RNA sequencing) and CITE-seq (cellular indexing of transc...
» CD8+ T cells in the cancer-immunity cycle
From pubmed: wherry ej[au]
CD8^(+) T cells are end effectors of cancer immunity. Most forms of effective cancer immunotherapy involve CD8^(+) T cell effector function. Here, we review the current understanding of T cell function in cancer, focusing on key CD8^(+) ...
» Prior vaccination promotes early activation of memory T cells and enhances immune responses during SARS-CoV-2 breakthrough infection
From pubmed: wherry ej[au]
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection of vaccinated individuals is increasingly common but rarely results in severe disease, likely due to the enhanced potency and accelerated kinetics of memory immune re...
» Author Correction: SARS-CoV-2 reservoir in post-acute sequelae of COVID-19 (PASC)
From pubmed: wherry ej[au]
No abstract
» SARS-CoV-2 reservoir in post-acute sequelae of COVID-19 (PASC)
From pubmed: wherry ej[au]
Millions of people are suffering from Long COVID or post-acute sequelae of COVID-19 (PASC). Several biological factors have emerged as potential drivers of PASC pathology. Some individuals with PASC may not fully clear the coronavirus SA...
» In vitro modeling of CD8+ T cell exhaustion enables CRISPR screening to reveal a role for BHLHE40
From pubmed: wherry ej[au]
Identifying molecular mechanisms of exhausted CD8 T cells (T(ex)) is a key goal of improving immunotherapy of cancer and other diseases. However, high-throughput interrogation of in vivo T(ex) can be costly and inefficient. In vitro mode...
» The pseudokinase Trib1 regulates the transition of exhausted T cells to a KLR+ CD8+ effector state, and its deletion improves checkpoint blockade
From pubmed: wherry ej[au]
CD8^(+) T cell exhaustion (T(EX)) impairs the ability of T cells to clear chronic infection or cancer. While T(EX) are hypofunctional, some T(EX) retain effector gene signatures, a feature associated with killer lectin-like receptor (KLR...
» Deep Phenotyping of the Lipidomic Response in COVID and non-COVID Sepsis
From pubmed: wherry ej[au]
Lipids may influence cellular penetrance by pathogens and the immune response that they evoke. Here we find a broad based lipidomic storm driven predominantly by secretory (s) phospholipase A (2) (sPLA (2) ) dependent eicosanoid producti...
» Phase I Trial of Autologous RNA-electroporated cMET-directed CAR T Cells Administered Intravenously in Patients with Melanoma and Breast Carcinoma
From pubmed: wherry ej[au]
CONCLUSIONS: Intravenous administration of RNA-electroporated cMET-directed CAR T cells is safe and feasible.
» The SWI/SNF chromatin remodeling complexes BAF and PBAF differentially regulate epigenetic transitions in exhausted CD8+ T cells
From pubmed: wherry ej[au]
CD8^(+) T cell exhaustion (Tex) limits disease control during chronic viral infections and cancer. Here, we investigated the epigenetic factors mediating major chromatin-remodeling events in Tex-cell development. A protein-domain-focused...
» The enteric nervous system relays psychological stress to intestinal inflammation
From pubmed: wherry ej[au]
Mental health profoundly impacts inflammatory responses in the body. This is particularly apparent in inflammatory bowel disease (IBD), in which psychological stress is associated with exacerbated disease flares. Here, we discover a crit...
» Signal recovery in single cell batch integration
From pubmed: wherry ej[au]
Data integration to align cells across batches has become a cornerstone of single cell data analysis, critically affecting downstream results. Yet, how much biological signal is erased during integration? Currently, there are no guidelin...
» High-throughput interrogation of immune responses using the Human Immune Profiling Pipeline
From pubmed: wherry ej[au]
The current abundance of immunotherapy clinical trials presents an opportunity to learn about the underlying mechanisms and pharmacodynamic effects of novel drugs on the human immune system. Here, we present a protocol to study how these...
» In Vitro Modeling of CD8 T Cell Exhaustion Enables CRISPR Screening to Reveal a Role for BHLHE40
From pubmed: wherry ej[au]
Identifying novel molecular mechanisms of exhausted CD8 T cells (T (ex) ) is a key goal of improving immunotherapy of cancer and other diseases. However, high-throughput interrogation of in vivo T (ex) can be costly and inefficient. In v...
» Efficient engineering of human and mouse primary cells using peptide-assisted genome editing
From pubmed: wherry ej[au]
Simple, efficient and well-tolerated delivery of CRISPR genome editing systems into primary cells remains a major challenge. Here we describe an engineered Peptide-Assisted Genome Editing (PAGE) CRISPR-Cas system for rapid and robust edi...
» Human T follicular helper clones seed the germinal center-resident regulatory pool
From pubmed: wherry ej[au]
The mechanisms by which FOXP3^(+) T follicular regulatory (Tfr) cells simultaneously steer antibody formation toward microbe or vaccine recognition and away from self-reactivity remain incompletely understood. To explore underappreciated...
» The pseudokinase Trib1 regulates the transition of exhausted T cells to a KLR + CD8 + effector state and its deletion improves checkpoint blockade
From pubmed: wherry ej[au]
T cell exhaustion (T (EX) ) impairs the ability of T cells to clear chronic infection or cancer. While exhausted T cells are hypofunctional, some exhausted T cells retain effector gene signatures, a feature that is associated with expres...
» Prior vaccination enhances immune responses during SARS-CoV-2 breakthrough infection with early activation of memory T cells followed by production of potent neutralizing antibodies
From pubmed: wherry ej[au]
SARS-CoV-2 infection of vaccinated individuals is increasingly common but rarely results in severe disease, likely due to the enhanced potency and accelerated kinetics of memory immune responses. However, there have been few opportunitie...